Hot start DNA polymerase for robust product formation.
•
Compatible with real-time, quantitative PCR and suitable for TA-cloning.
SPECIFICATIONS
•
Storage: Store at -20°C for up to 12 months. Avoid repeated freeze/thawing of reagents. Prolonged storage is at -80°C.
•
Enzyme Concentration: Reaction conditions at 1X (50 µl total volume) will contain 2 U of ZymoTaq™ DNA polymerase. As the ZymoTaq™ PreMix (2X), the enzyme is at 4 U/50 µl.
•
Unit Definition: > One unit (U) enzyme is defined as the amount of enzyme required for the incorporation of 10 nM dNTPs into an acid-insoluble form in 30 minutes at 72°C.
DESCRIPTION
ZymoTaq™ DNA Polymerase is specifically designed for the amplification of “difficult” DNA templates including: bisulfite-treated DNA for methylation detection, real-time and quantitative PCR that are SYBR Green and probe based. The product generates specific amplicons with little or no by-product formation. Simple and easy to use... just heat at 95°C for 10 minutes to activate polymerase activity.
ZymoTaq™ DNA Polymerase is available either as a single buffer premix or as a polymerase system with components provided separately.
Efficient PCR amplification of bisulfite treated DNA for methylation detection. The figure shows 200 bp product formation from bisulfite-treated DNA using ZymoTaq™ PreMix versus the polymerase systems from Suppliers R, Q, S, and I, respectively. In each case, equal amounts of bisulfite-treated DNA (EZ DNA Methylation-Direct™ Kit from Zymo Research) were used for each PCR, and the products separated in a 2.0% (w/v) agarose/TAE/EtBr gel. Also evident from the image, is the low occurrence of both primer dimerization and non-specific product formation.
