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ZR Plasmid Miniprep™-Classic
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For purification of high quality, endotoxin-free plasmid DNA for restriction endonuclease digestion, DNA sequencing, transformation, cloning, transfection, in vitro transcription reactions, etc. |
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Unique column design: zero buffer retention and low volume (30 µl) elution. |
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Innovative colored buffers for error-free visual identification of complete bacterial cell lysis and neutralization. |
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Format: Spin Column |
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Plasmid DNA Yield: Up to 25 µg per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. coli utilized. |
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Plasmid DNA Size: Up to 25 kb. |
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Recovery Volume: ≥30 µl |
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DNA Purity: Plasmid DNA which is well suited for ligation, sequencing, restriction endonuclease digestion, in vitro transcription, and other sensitive applications requiring pure DNA. Typical A(260/280) index is ≥1.8. |
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Procedure: Performed at room temperature (15-30°C) using a centrifuge and/or a vacuum manifold. |
The ZR Plasmid Miniprep™-Classic kit is designed for efficient isolation of plasmid DNA from E. coli cell lysates using a procedure that is simple, rapid, user-friendly, and reliable compared to the products offered by the competition. It features a modified alkaline lysis protocol together with a unique Fast Spin column to yield high quality plasmid DNA in minutes. The ZR Plasmid Miniprep™-Classic features color-coded (red, blue, yellow) reagents for easy determination of complete cell lysis. The innovative Zymo-Spin™ llN columns facilitate high yield plasmid DNA that is endotoxin-free. Plasmid DNA purified using the ZR Plasmid Miniprep™-Classic kit is well suited for use in restriction endonuclease digestion, sequencing, DNA ligation, cloning, PCR, bacterial transformation, transfection, etc.
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