For high quality DNA purification from solid tissues (e.g., tailsnips, earpunches, adipose tissue, etc.), whole blood, plasma, serum, buffy coat, lymphocytes, cultured cells, FFPE tissues, semen, hair, and other biological sources. Up to 125 µg/prep.
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Combines Proteinase K digestion with innovative Fast-Spin column purification technology.
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Isolated DNA is ideal for PCR, endonuclease digestion, Southern blotting, bisulfite conversion/methylation detection, sequencing, genotyping, etc.
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Sample Sources: Solid tissues (e.g., tailsnips, earpunches, adipose tissue, etc.), whole blood, plasma, serum, buffy coat, lymphocytes, cultured cells, FFPE tissues, semen, hair, and other biological sources are effectively processed using this kit.
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DNA Purity: High quality DNA for PCR, endonuclease digestion, Southern blotting, bisulfite conversion/methylation detection, sequencing, genotyping, etc., is eluted with DNA Elution Buffer or water. (A260/A280 ≥1.8)
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DNA Size: Capable of recovering genomic and mitochondrial DNA sized fragments from 100 bp to ≥ 40 kb. If present, parasitic, microbial, and viral DNA will also be recovered. Typical fragment sizes range from 25 kb-35 kb.
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DNA Yield: The DNA binding capacity of the column is 125 µg. Typically, mammalian tissues yield: 1-3 µg DNA per mg skeletal, heart, and brain tissues and 3-5 µg DNA per mg liver, kidney and lung tissues. Human whole blood will yield 3-7 µg DNA per 100 µl blood sampled. DNA is eluted into ≥150 µl DNA Elution Buffer or water.
Equipment: Water bath or heat block (55oC), centrifuge or vacumm source and manifold, microcentrifuge, and vortex.
The ZR Genomic DNA™-Tissue MidiPrep is a straightforward procedure for the rapid isolation of total DNA (e.g., genomic, mitochondrial, parasitic, microbial, viral) from a variety of solid tissues that are either fresh, frozen, or FFPE. This product has been optimized for maximal recovery of ultra-pure DNA without RNA contamination and is also compatible with buffy coat, bone marrow, cells from culture, whole blood (fresh or stored), serum, plasma, and many biological liquid samples. For processing, simply digest the sample with the supplied Proteinase K then add the Genomic Lysis Buffer, vortex, and transfer the mixture to the supplied Zymo-Spin™ column/filter assembly. PCR inhibitors are effectively removed during the purification process and purified DNA is suitable for downstream applications including: PCR, Southern blotting, DNA sequencing, endonuclease digestion, bisulfite conversion/methylation analysis, etc.
