EZ DNA Methylation-Direct™ Kit (Free Kit Offer)  HIGHLIGHTS • Complete bisulfite conversion of DNA directly* from blood, tissue, or cells. • Compatible with small sample inputs – as few as 10 cells or 50 pg DNA. • Well-suited for FFPE and LCM-derived samples.

SPECIFICATIONS   • Cells: Compatible with cells from solid tissue, tissue culture, whole blood, buffy coat, biopsies, LCM (Laser-Capture Micro-Dissection) and FFPE samples, etc. The number of cells per standard treatment can range from 10 - 105 cells. For optimal results, the cell number should be from 1 x 103 - 8 x 104 cells. • Purified DNA: Samples containing 50 pg - 2 µg of DNA. For optimal results, the amount of input DNA should be from 200 to 500 ng. • Conversion Efficiency: > 99.5% of non-methylated C residues are converted to U; > 99.5% protection of methylated cytosines. • DNA Recovery: > 80% • Sensitivity of Detection (Lower Limit): 10 cells for successful PCR amplification.

DESCRIPTION The EZ DNA Methylation-Direct™ Kit is a further refinement of our popular EZ DNA Methylation™ and EZ DNA Methylation-Gold™ Kits. The EZ DNA Methylation-Direct™ Kit features simple and reliable DNA bisulfite conversion directly from blood, tissue, and cells without the prerequisite for DNA purification. The increased sensitivity of this kit makes it possible to amplify bisulfite converted DNA from as few as 10 cells or 50 pg DNA. Like the EZ DNA Methylation-Gold™ Kit, DNA denaturation and bisulfite conversion processes are combined into a single step (see below). All kits streamline the three step process of bisulfite conversion of non-methylated cytosine in DNA into uracil. In addition the methylation kits share innovative in-column desulphonation technology that eliminates otherwise cumbersome DNA precipitation steps while ensuring researchers consistent results every time. The kits have been designed to minimize template degradation, loss of DNA during treatment and clean-up, and to provide complete conversion of unmethylated cytosines. Recovered DNA is ideal for PCR amplification for downstream analyses including restriction endonuclease digestion, sequencing, microarrays, etc. An outline comparing the EZ DNA Methylation-Direct™ Kit procedure to Zymo Research’s other methylation kits is shown below.